Study on multidrug-resistant(MDR) uropathogenic Escherichia coli (UPEC) isolates, presence of beta lactamase genes and screening of them for O25b/ST131 strain in Tabriz 2020

Abstract

The presence of the O25b-ST131 clone and the production of extended-spectrum beta-lactamases (ESBL) all of the world has become common, often associated with multidrug resistance. Aim of the present study, investigating the presence of beta-lactamase genes among drug-resistant uropathogenic Escherichia coli and screening of O25b-ST131 strain in urine cultures of outpatients and inpatients in Tabriz. Method and materials: In this study, 150 non-repetitive isolates of Escherichia coli from outpatients and inpatients with urinary tract infection referred to Asadabadi and Sina Tabriz hospitals over the years 1399-1400 were collected. Demographic and clinical data of patients were extracted. For determining the pattern of antibiotic resistance using disk diffusion agar method and to determine the minimum concentration Colistin inhibitor was used by microbroth dilution method. Beta-lactamase genes and O25b-ST131 clone were identified by PCR. Results: Escherichia coli isolates were sensitive to the meropenem (99.3%) and fosfomycin (98.7%) antibiotics but they had highly resistant to amoxicillin (81.3%) and cotrimoxazole (62%). Among beta-lactamase genes, the highest rate is related to blaCTXM (46%) and the lowest rate is related to blaSHV (20%). In all the isolates, O25b-ST131 clone (86%) had the highest frequency.