Investigating The Effect of Double Silencing of STAT3 and B-catenin in 4T1 and CT26 Cancer Cells

Abstract

Due to the presence of complex compensatory signaling pathways in the tumor microenvironment، monotherapy fails in most cases، and many researchers are looking for effective combination treatments against cancer. One of the most important signaling pathways in the tumor microenvironment is the STAT3/B-catenin pathway, which synergistically causes the unbridled growth of cancer cells. Considering the additive effect that these two factors have on each othe, the combined targeting of this pathway can be considered a new method in cancer treatment. Therefore، in this study, it was decided to target the STAT3/B-catenin pathway using nanoparticles loaded with siRNA. Materials and methods: In this study، STAT3/B-catenin pathway was targeted using chitosan-based nanoparticles loaded with siRNA. This study was conducted on two mouse cell lines including 4T1 and CT26. Examining the expression of target genes was done using Real-time PCR technique. Studying the effect of treatment on cell viability was done by MTT method. Findings: The result of this research showed that nanoparticles loaded with siRNA significantly decrease the expression of STAT3/B-catenin genes and tumor promoting factors. Also، targeting the STAT3/B-catenin pathway can cause the death of cancer cells.