Evaluation of LINC01111, miR-3924 and DUSP1 expression levels In tumor samples and healthy margins of SCC of the tongue

Abstract

Squamous cell carcinoma (SCC) is the second most common skin malignancy after basal cell carcinoma, and its prevalence is increasing worldwide. Eighty-five to ninety-five percent of all cancers are malignant squamous cell carcinoma (SCC), which is very dangerous and deadly. The effective factors in causing cancer are different. Some of these factors can be completely removed from life, for example, alcohol consumption can increase the chance of developing this malignancy, and by removing it, the development of this malignancy can be prevented. The stable reduction of LINC expression enables these cells to have a higher ability in cell proliferation, metastasis and migration in laboratory conditions, and also the reduction of the expression of this gene in tumors increases tumor growth and metastasis in vivo. For this reason, we decided to investigate The relationship between the expression level of LINC-01111, DUSP1 and MIR-3924 in SCC tumor samples compared to its margin.

Method: In the present study, 50 samples of tumor tissue and healthy margin of tongue SCC specimens purchased from the National Bank of Alborz Province. After receiving the samples from the relevant center using RNA extraction kit made by Qiagen company, the samples extracted and after cDNA synthesis using Takara synthesis kit, the amount of gene expression evaluated.

Results: while expression levels of LINC-01111 are significantly higher in SCC patients, the expression levels of DUSP-1 and miR-3924 are significantly lower, Also, these expression changes in some cases were related to the clinicopathological characteristics of the patients.