The role of miR-526p in proliferation and susceptibility of osteosarcoma cancer cells in the presence of paclitaxel

Abstract

Recently, various studies have focused on the therapeutic potential of miRNAs, especially miR-526, which is one of the tumor suppressor miRNAs, in various human malignancies. However, the underlying mechanisms in the anticancer effects mediated by miR-526 are still not fully understood. Therefore, the present study investigated the effect of miR-526 on drug resistance and apoptosis caused by paclitaxel in MG-63 cells. Methods: Sensitive and resistant MG-63 cells were treated with miR-526 mimetic and inhibitor, paclitaxel and a combination of both, and cell viability was evaluated by MTT method. miR-526 expression was assessed using quantitative real-time polymerase chain reaction (qRT-PCR). The expression levels of P-gp and PTEN were evaluated using Western blot. Flow cytometry method was also used to investigate apoptosis. Results: Paclitaxel resulted in significant inhibition of cell proliferation in a dose-dependent manner. The combination of miR-526 and paclitaxel resulted in a significant inhibition of cell proliferation compared to single treatments (P<0.05). miR-526 also decreased P-gp expression and increased PTEN expression. Furthermore, miR-526 increased paclitaxel-induced apoptosis in MG-63 cells.