Phytochemical investigation of Vitis vinifera leaves Raised in Malayer

Abstract

Introduction: Vitis species are widely used in traditional medicine. Considering the relationship between the chemical compounds in plants and their observed effects, there is a need to investigate and identify the effective compounds of plants.Purpose : Until now, the attention of researchers has been focused on the grape itself and its core, and the grape leaves were often thrown away by farmers. This happens while grape leaves are a rich source of polyphenolic compounds and antioxidants. Considering the limited studies on the leaves of Vitis vinifera grape plant and the lack of domestic production of medicinal products from grape leaves, extracting and investigating the phytochemical compounds in the leaves of local grape cultivars will be innovative.Methods and materials: A sufficient amount of leaves of the plant were collected in the summer season from private farms in Malayer city (Hamadan province) and dried in the shade after washing. Dry samples were extracted by powder grinding and then by n-hexane, chloroform and methanol solvents and with the help of Soxhlet . Then their solvent was evaporated by the evaporator. The methanolic extract was fractionated and separated by various methods of solvent-to-solvent extraction and chromatography (column chromatography), and finally five pure substances were identified by NMR.Results: The following compounds were identified from the ethyl acetate fraction of the methanolic extract of this plant: rosmarinic acid 2 methyl quercetin 3-O-β-D glucopyranoside Quercetin-3-O-β-D-glucuronide 6′′-methylester phenyllactic acid kaempferol-3-O-β-D-glucuronide Conclusion: Phytochemical study on the ethyl acetate fraction of Vitis vinifera led to the isolation of five compounds. The main compound of the ethyl acetate extract of the plant is Quercetin-3-O-β-glucopyranoside, which has strong antioxidant and anticancer effects.