Single nucleotide polymorphism of TAP1 and TAP2 genes in patients with hydatidosis

Abstract

Hydatid cyst is a neglected parasitic disease that occurs in the larval stage of the canine tapeworm Echinococcus granulosus and can cause irreparable damage and expensive treatments to affected individuals. Although most species of parasites can cause disease in humans, a healthy and efficient immune system plays an important role in controlling and preventing the spread of infections. Single nucleotide polymorphisms (SNPs) on the TAP1/637 and TAP2/379 genes may play a role in hydatid cysts by affecting the immune system. Materials and Methodes: This study were conducted as a case-control study. Blood samples were collected from 76 individuals with hydatid cyst after confirmation by the serological ELISA method and also from 76 healthy individuals. For molecular analysis, DNA was extracted from the samples and the ARMS-PCR technique was used to identify mutations. Finally, the PCR product was subjected to electrophoresis on a 2% agarose gel and the bands were analyzed under UV light. To evaluate the accuracy and ensure the PCR results, some samples were also sequenced in two groups of patients and controls with different genotypes. Results: In this study, the frequency of heterozygous Asp/Gly codon 637 of the TAP1 gene was higher in the patient group than in the control group (OR=3. 255, P=0. 004). The frequency of the phenotype and Gly allele was also higher in the patient group than in the control group (P<0. 05). In addition, the frequency of heterozygous Val/Ile codon 379 of the TAP2 gene was higher in the patient group than in the control group (OR=2. 492, P=0. 009). The frequency of the phenotype and Ile allele was also higher in the patient group than in the control group (P<0. 05).