Detection of genesencoding enterotoxin types SEQ,SEA,SEC in staphylococcus aureus isolates from clinical samples by CPR

Abstract

Staphylococcus aureus is a major cause of hospital-acquired and community-acquired infections, leading to a high mortality rate. Staphylococcus aureus enterotoxins, responsible for approximately 50% of food poisoning cases in humans, are significant virulence factors. The aim of this research is to isolate Staphylococcus aureus bacteria and identify strains carrying genes encoding enterotoxins types A, C, and Q from clinical samples of patients using PCR. Materials and Methods: During the study, 96 isolates of Staphylococcus aureus were obtained from clinical samples of patients at Shohada Modarres Hospital in Tabriz. These isolates were transferred to the microbiology laboratory and identified using routine microbiological methods. The antibiotic sensitivity of Staphylococcus aureus isolates to seven different antibiotics was determined using the disc diffusion method and following CLSI standards. Genomic DNA extraction from isolates was performed using the boiling method, followed by PCR to detect sea, sec, and seq genes. Results: Out of the total 96 Staphylococcus aureus isolates, the highest resistance was observed to clindamycin at 59%, while the lowest resistance was to vancomycin, with all isolates being sensitive to this antibiotic. Resistance to other antibiotics included erythromycin and linzolid at 54%, cefoxitin at 51.04%, gentamicin at 44%, and cotrimoxazole at 16%. PCR results indicated that 79.19% of isolates carried the sea gene, while the isolates studied were devoid of sec and seq genes.