Preparation of Lipid based Nanoparticles from Extracts of Grape seeds and Evaluation of Their In Vitro Anticancer Effect

Abstract

Oxidative stress is recognized as one of the important factors in the occurrence and progression of cancer. In recent years, the unique antioxidant activity of grape seed extract has compelled researchers to evaluate its effects in various diseases and observe positive impacts on cardiovascular diseases, diabetes, and certain types of cancer. Nano-lipid carriers are biocompatible nanoparticles with high cellular uptake, chosen for delivering this herbal extract. Objective: This study aimed to investigate the effect of grape seed extract delivering by nano-lipid carriers on the cytotoxicity of this extract in vitro.Materials and Methods:After selecting the best extraxt of grape seeds, the obtained natural product was loaded into nano-lipid carriers. Morphology and drug loading capacity were examined, and MTT assay was conducted on cancer cell lines to determine cell viability and IC50. Results:By checking the weight of the dried samples after extraction and checking their cellular cytotoxicity, methanol solvent was used as the solvent of choice for extraction step. Nanocarriers loaded with drugs have high antioxidant activity, considerable stability, and the results of the MTT tests clearly show a decrease in IC50 and an increase in cytotoxicity following loading of GSE in nanoparticles.Conclusion:The loading of GSE in the NLC nanocarrier increased the cytotoxic properties of this extract and caused it to show its toxic effects on cancer cells at a much lower dose. It is worth mentioning here that the investigation of the cytotoxic properties of the nanocarrier in this experiment cannot be investigated due to the unavailability of reliable data.