Correlation of common polymorphisms of the gene encoding Nav1.8 sodium ion channel with the success of inferior alveolar nerve block in mandibular molars with symptomatic irreversible pulpitis

Abstract

Objectives: This study assessed the correlation of polymorphisms (rs6801957 and rs6795970) of the SCN10A gene encoding Nav1.8 with the success of inferior alveolar nerve block (IANB) in mandibular molars with symptomatic irreversible pulpitis. Materials and Methods: This cross-sectional study was conducted on 194 patients (107 females, 87 males) requiring endodontic treatment of mandibular first or second molars with symptomatic irreversible pulpitis. Endodontic treatment was started 10 minutes after anesthesia of the lip and no response to cold test and electric pulp test. The pain intensity was quantified using a visual analog scale (VAS). Saliva samples underwent DNA extraction, and

polymorphisms were evaluated by the Tetra ARMS-polymerase chain reaction (PCR). Data were analyzed by the Chi-square test, independent samples t-test, and logistic regression (alpha=0.05). Results: In rs6801957 polymorphism, the failure rate in males (TG>TT,GG), and the failure rate of females (TT >GG,TG) and in males and females, the failure rate of (G >T) was observed. In rs6795970 , the failure rate in males and females (AG>AA,GG), and the failure rate in males and females(G>A). Conclusion: The rs6795970 polymorphism of the SCN10A gene significantly affected the success of IANB in mandibular molars with symptomatic irreversible pulpitis. different genotypes of this gene are probably at higher risk of IANB failure.