Analysis of the effect of human mesenchymal stem cells on regulatory T cells in patients with chronic obstructive pulmonary disease (COPD)

Abstract

Recent studies have shown that inflammatory responses play a significant role in the development and progression of COPD. So far, various treatment methods have been suggested for the treatment of these patients, but there is no definite and perfect treatment. In this study, we investigated the regulatory effects of MSC cells on the activity of peripheral blood Treg cells of patients with COPD in a laboratory environment. Materials and methods: after isolation of peripheral blood mononuclear cells (PBMC), the cells were cultured and the level of gene expression and secretory level of cytokines (IL-35, IL-10 and TGF-β) as well as the abundance of cells Tregs were measured. Then, in the next step, peripheral blood mononuclear cells were co-cultured with human MSC cells and all the mentioned factors were measured after co-culture. Finally, the amount of changes was statistically analyzed with statistical methods. Results: The results of the present study showed that mesenchymal stem cells increase the differentiation of regulatory T cells. Also, the results showed that human MSCs can significantly increase the gene expression and secretory levels of IL-35, IL-10 and TGF-β cytokines in PBMC of people with COPD.