| dc.description.abstract | Infectious diseases are one of the biggest global health challenges. Urinary tract infection (UTI) is the second most common clinical disease and imposes a heavy economic burden on the healthcare system. Meanwhile, common antibiotic treatments are associated with the development of resistance. The use of green synthesis methods by researchers is rapidly increasing due to the use of less toxic and environmentally friendly chemicals and also due to the one-step synthesis of nanoparticles. Based on this, in this project, the antimicrobial effects of selenium nanoparticles obtained from green synthesis on common pathogens causing urinary tract infections have been investigated.
Methodology and Materials: For the preparation of selenium nanoparticles prepared by the green method, the extract of elderberry plant and its combination with sodium selenite solution were used. The size of nanoparticles, morphology, surface charge, functional groups and possible interactions about the physicochemical properties of nanoparticles were among the evaluation goals of this research. The amount of MBC was determined with the help of the contents of the microplate wells of the agar medium in which the MIC test was performed. After obtaining the MBC of the nanoparticles, their antimicrobial effects were evaluated using the Time Kill Kinetics method. Finally, the disc diffusion process was performed in order to evaluate the sensitivity of bacteria to SeNPs.
Results: Nanomaterials are mostly spherical and nano-sized and are in the form of clusters that are not in direct contact with each other, and this confirms the stabilization and stability of selenium nanoparticles. The average particle size in the present study was reported to be 85 nm, and the findings indicated that the particle size distribution showed monodisperse particles. There was no significant difference between SeNPs and control antibiotics (Amikacin and Ciprofloxacin) in inhibiting the growth of E. coli and K. pneumonia bacteria, respectively. In fact, the antibacterial effect of SeNPS against E.coli is similar to amikacin, but compared to ciprofloxacin, it has a weaker antibacterial effect against K. pneumonia. Regarding the result of the kinetics of bacterial death, selenium nanoparticles showed favorable effects on both bacteria, and in this case, the effect was faster on E. coli. | en_US |
