Evaluation of CD35 molecules on red blood cells and TCR lymphocytes in patients with lupus erythromatosis

Abstract

Systemic Lupus Erythematosus (SLE) is an autoimmune disease, in the investigation of its pathological mechanism, several disorders in the immune system, including interference with the production of cytokines, have been described. However, the exact mechanisms of its damage are still unclear and the cytokines involved are not fully understood. Cytokines are multifaceted in their biological activities and our immunity is clearly regulated by the complex cytokine network. Therefore, the role of these abnormal cytokines and regulatory networks of these molecules in the pathological course of lupus disease is very important in order to plan diagnosis and treatment. Material and methods: the number of 40 controls and 35 lupus patients referred to Sina Hospital by dermatologists and hair samples and 10 cc blood medium of healthy controls and people with lupus in sterile heparin tubes in April. And the RBCs were separated by Ficoll and one million RBCs were added to the tube for each tube. 4 ml of monoclonal anti-CD35 antibody with 1 mg/ml was added to the tubes and incubated, and after adding the secondary antibody, they were centrifuged.It was written and analyzed by flow cytometer. Polyethylene globule method was used to measure immune complexes. The data was analyzed by SPSS18 and one-way t-student ANOVA. The sample size was determined by Cochran's formula. Results: The results obtained from this study show that there is a relationship between the disease and the expression of CD35, C3, C4 molecules. The molecular expression of CD5 is decreased in patients compared to healthy individuals. Also, the expression of C3 is reduced in relation to the healthy mean, but not to a high level.