Investigation of the cytotoxic effects of green synthesis selenium nanoparticles derived from Elaeagnus Angustifolia L in prostate cancer cell line (PC-3)

Abstract

Prostate cancer is the second most common cancer in men and the fifth cause of death in the world. According to estimates in 2018, about 1,276,000 new cases of prostate cancer were reported worldwide. Materials with nano dimensions have attracted a lot of attention from medical researchers in recent years. Metal nanoparticles have been widely used in medical and non-medical fields in biology in recent years. Among the metal nanoparticles, selenium nanoparticles have been widely investigated due to their distinctive characteristics, such as good electrical conductivity, chemical stability, catalytic and antibacterial activity. Investigating the possible effects of inhibiting the growth of prostate cancer cells in the PC-3 cell line. Materials and Methods: The current research was an experimental study in which prostate cancer cells (PC-3 cell line) were used. PC-3 cell line was purchased from Pasteur Institute, after cell culture and proliferation, these cells were treated with different concentrations of green selenium nanoparticles. Then MTT tests were used to check cell viability and Trypan blue exclusion test was used to check cell lethality and the amount of reactive oxygen species was also checked. Results: In this study, 50IC of green selenium nanoparticles in PC-3 cell line was measured as 81.33 ± 7.32 μg/ml and R2=0.92. The cell viability of PC-3 cells treated with concentrations of 40, 80, 100 μg/ml of green selenium nanoparticles was significantly decreased compared to the control group (P<00.1). The production of reactive oxygen species (ROS) caused by selenium nanoparticles increased significantly compared to the control group (P<0.001).