Comparison of TGFB, IL1 production in peripheral blood monocytes of patients with multiple sclerosis with healthy individuals

Abstract

An autoimmune disease is a disorder in which nerve cells in the brain are damaged. This damage can affect the nervous system and cause many physical signs and symptoms. B plays a role in the repair of nervous tissue and can be prescribed in neurodegenerative diseases and cerebral ischemia. IL-1 is a glycoprotein that is involved in hematopoiesis and the production of cytokines. It also plays a role in the movement of myelin clearance by the brain's innate immune cells, which is a prerequisite for proper remyelination and myelin repair. The aim of this study is evaluation of inflammation and inflammatory factors in MS. Materials and methods: Cytokine IL-1, TGF-B will be measured inside cells by flow cytometry and in plasma by ELISA. In the special flow cytometry evaluation, peripheral blood will be prepared from 17 patients with MS (14 women and 3 men) and 17 healthy people (14 women and 3 men) as control samples. 100 microliters of the peripheral blood of a healthy or sick person will be moved with LPS and leptin for different periods of time, and then the cells will be fixed with 4% paraformaldehyde and permeabilized with 0.05% saponin. At the end, the cells will be stained with anti-TGF-B, IL-1α antibody and flow cytometry analysis. Data analysis will be done with ONE WAY anova and SPSS software. Results: IL-1 production is higher in MS patients than healthy people, and TGF-B production is higher in MS patients than healthy people.