Preparation and characterization of OVA loaded CS-PEG/PLA/PCL nanoparticles for protein vaccin delivery

Abstract

The use of nanotechnology in vaccine field has provided an opportunity to create effective vaccines. In particular, nanoparticles used as carriers of vaccine components are able to enhance host immune responses and reach specific cellular regions due to their size. To date, few nanovaccines have been approved for humans, and many have been studied in clinical or preclinical trials. Nano-vaccines have been extensively explored to induce a robust immune response by the advantages including nano-size range, high antigen loading, enhanced immunogenicity, presentation of antigen on MHC class I molecules, and promotion of patient acceptance with decreasing booster doses, and have great potential for the prophylactic and therapeutic uses (1).Target:Preparation biodegradable nanoparticles encapsulated OVA model antigen and characterization of physicochemical properties and cytotoxicity with the approach of providing a new model for nano-vaccines .Method :OVA loaded Chitosan-PEG-PLA-PCL nanoparticles were prepared by double emulsion solvent evaporation method and coated with chitosan. The chemical structure of the copolymer was studied by FT-IR spectroscopy. Size distribution and surface charge of particles and their morphology were studied by DLS and SEM experiments.The release of OVA from nanoparticles at the temperature and pH of blood was done by the sample separate method. MTT test was performed to check the cytotoxicity of nanoparticles for cells.Results :FT-IR spectrum results confirmed the present functional groups. The results showed that coat of chitosan can increases the size of nanoparticles, positive surface charge and increases the percentage of encapsulation efficiency and controls the release of OVA from nanoparticles. MTT test results showed that nanoparticles are not toxic for cells.Conclusion :The prepared nanoparticles have potential for novel model of effective nanovaccines