The role of miR-100 in methotrexate resistance in MG-63 osteosarcoma cell line

Abstract

Recently, various studies have focused on the therapeutic potential of miRNAs, especially miR-100, which is one of the tumor suppressor miRNAs, in various human malignancies, including osteosarcoma. However, the underlying mechanisms in miR-100-mediated anticancer effects are still not fully understood. Therefore, the present study investigated the effect of miR-100 on methotrexate (MTX)-induced apoptosis in MG-63 cells. Methods: MG-63 were treated with MTX, miR-100 and a combination of both, and cell viability was evaluated by MTT method. The expression of miR-100, tetrahydrofolate reductase was evaluated using quantitative real-time polymerase chain reaction (qRT-PCR). Flow cytometry method was also used to investigate apoptosis. Results: Overexpression of miRNA-205 was shown to significantly inhibit the viability of MG-63 cells. Overexpression of miR-100 led to decrease Tetrahydrofolate reductase expression. Also, overexpression of miR-100 increased the apoptosis rate of MG-63 cells.