Study on the effect of azithromycine pretreatment on 7, 12-Dimethylbez (a)anthracene and croton oil induced skin tumorigenesis in the mice

Abstract

Cancer incidence is one of the major problems of todays world. several studies have been performed to determine the exact mechanism and its protective and therapeutic factors. Objective:This study is aimed to investigate the local protective effect of Azithromycine solution on skin cancer induced by dimethylbenz(a)anthracene and croton oil .Methods & Materials:Eighty female Albino Swiss mice were divided into 4 groups of 20 in each group. The back of the mice were shaved. After 24 hours, the animals of group 0 received Placebo gel as control and group 1,2,3 respectively Azithromycine 2% ,4% and 8% were administered. The medication is applied topically once a day for 15 days. Nine hours after application of the final dose, all groups were provided by a single dose of DMBA (60g/100l acetone/mouse) and After 7 days, croton oil (1mg/200l acetone/mouse) applied twice weekly for period of 28 weeks. Results:Inaddition to fewer tumor number in the groups receiving azithromycine, a significant delay was also observed in tumor formation proved by P Value. Discussion and Conclusion: Results have shown that Azithromycine can reduce the count and decelerate the growth rate of skin tumors compared to the control group. Generally this protective and inhibitory effect of Azithromycine in the induction of skin cancer in mice its potential ability to impede oxidative stress and DNA damage. Overall, our study demonstrates that Azithromycine in high doses is responsible for inhibition of mouse skin carcinogenesis. However, the exact mechanism remains elusive and needs further experimentations.