Study of the pattern of angiogenic genes and MiRs controlling their expression in colorectal cancer treated with cordycepin

Abstract

Genetic changes play an important role in the development of gastric cancer. In addition to changes in the level of DNA, changes in the expression level of MicroRNAs are also important in cancer progression. It is understood that the intercommunication between cancer cells and their microenvironment is essential to tumor angiogenesis. The aim of this study was to investigate the expression of TGF-β, HIF-1α genes and miRNAs that control them in treatment with cordycepin. Material and Methods: HT-29 colorectal cancer cell line was cultured in the relevant culture medium and after treatment with TLA antigen, RNA was extracted. Then cDNA was synthesized. Finally, changes in the expression of TGF-β, HIF-1α genes and their control miRNAs (miR-199 and miR-342) were determined by qRT-PCR. Results: QRT-PCR test changes in the expression of HIF-1α gene significantly increase was observed but expression of TGF-β significantly decreased. About miRs controlling them, miR-199 and miR-324, in treatment with cordycepin showed a significant decreased.