Evaluation of Valproic acid function on TLR2,TLR4,TLR9 expression on monocyte cells from MS patients

Abstract

MS is an inflammatory disease in which the myelin sheaths of nerve cells in the brain and spinal cord are damaged. This injury can disrupt the ability of parts of the nervous system that are responsible for communication and cause many physical signs and symptoms. In addition to blood cells and epithelial and endothelial cells, TLRs are also present in the brain. The reason for choosing TLR2 and TLR4 is due to the great importance of these 2 receptors in the transmission of messages at the cell level and the membrane level. Materials and methods: 30 MS patients referred to the MS clinic of Imam Reza Hospital with mild, moderate and severe stages were selected, and blood samples were taken from the patients, and the monocyte cells of the MACS brush were separated from the PBMC. was with CD14 antibody. Monocyte cells were then cultured and stimulated with valproic acid as a time course of 0, 6, 12, 24 and 48 hours. After the end of stimulation, the cells were collected and the expression level of TLR9, TLR2, TLR4 was evaluated by Real Time PCR and Western Blot. Results: The expression level of TLR2, TLR4 and TLR9receptors increased significantly with the passage of time as a time course of stimulation, and this increase reached its maximum value 12 hours after stimulation.