Comparison of the Effect of Mesenchymal Stem Cells with Cerebrolysin Loaded Nanoparticles on Expression of Immune Cell’s Transcription Factors, MMP9 and IFN-γ in EAE an Animal Model of Multiple Sclerosis in C57BL/6 mice

Abstract

Introduction: Multiple sclerosis (MS) is classified as an autoimmune disease affecting the central nervous system (CNS). Administration of bone marrow-derived mesenchymal stem cells (BMSCs) indicates alleviation of symptoms in animal models of MS. Cerebrolysin (CBL) loaded TGN-modified chitosan nanoparticles (CBL@TCN) could represent a promising approach to significant reduction in experimental autoimmune encephalomyelitis (EAE) symptoms. Methods and materials: This study aims to explore behavioral and biochemical variations in an acute stage of MS, comparison the efficiency of BMSCs with CBL@TCN. C57BL/6 female mice receiving myelin oligodendrocyte glycoprotein (MOG35-55) via hind flank and intraperitoneally (IP) pertussis toxin after a short time were separated in the negative control group receiving Phosphate-buffer saline (PBS) (n=8), the positive control mice EAE (n=8), the EAE receiving CBL (5 mg/kg/day) as standard treatment, the EAE receiving CBL@TCN (0.5 mg/mouse on the 13th and 19th days after immunization) (n=8), and the EAE receiving BMSCs group (2×106 cell/mouse) (n=8). All administration injected IP. Results: The clinical scores considerations and evaluation of biological samples showed significant improvement in locomotion activity accompanied by a decrease in lymphocyte infiltration on spinal cord tissues in the CBL@TCN received group. In conclusion, CBL@TCN in comparison with other groups provided evidence of regenerative mechanisms by which promoted morphofunctional improvement by activation of survival pathways after induction of EAE in a preclinical model of MS.