The effect of Toxoplasma gondii lysate antigens on angiogenesis in brain cancer cell line

Abstract

Objective: T. gondii is an obligatory protozoan parasite that is the most common zoonotic parasite worldwide. This pathogen can infect almost all warm-blooded animals and humans. In healthy people, the parasite can remain asymptomatic. In immunocompromised patients, the parasite can become reactive and cause severe complications such as encephalitis. Aim of the study is the effect of T. gondii on angiogenesis in U373 cell line.Material and Methods: The cell line is cultured in RPMI1640 medium and after the cells reach logarithmic growth, two groups with/without T. gondii lysate treated will be considered. After 24 hours, the cells will be collected for mRNA extraction, and after cDNA synthesis. The expression of the genes examined by RT-PCRResults: QRT-PCR test changes in the expression of VEGF, MMP9 genes significantly increased in 20 μg/ml of T. gondii but strikingly decreased in 80 μg/ml.