Assessment and comparison of urinary levels of 8-hydroxy-2-deoxy guanosine and F2a-isoprostane in male football players and healthy non-athlete controls, and their association with dietary inflammatory index and dietary total antioxidant capacity

Abstract

Results: We found that, the levels of 8-OHdG (β=-8.25; P<0.001) and F2a-IP (β=-98.46; P<0.001) were significantly lower in the football player group than in the non-athlete group. In addition, football player group in comparison to the non-athlete group had lower DII score (β=-2.06; P<0.001), and higher FRAP (β= 2.37; P<0.001), ORAC (β=4.36; P<0.001) and TRAP (β=3.44; P<0.001) scores. In all participants, dietary indices were significantly related to oxidative biomarkers. Positive associations were found between DII with 8-OHdG (β=2.25; P<0.001) and F2a-IP (β=28.34; P<0.001). Furthermore, negative associations between FRAP (β=-1.42; P<0.001), ORAC (β=-0.67; P<0.001) and TRAP (β=-0.78; P<0.001) with 8-OHdG were observed. In addition, FRAP (β=-17.34; P<0.001), ORAC (β=-8.08; P<0.001) and TRAP (β=-9.28; P<0.001) were negatively associated with F2a-IP. Conclusion: In the present study, the levels of urinary biomarkers for DNA and lipid oxidative damages were lower in football players compared to non-athlete controls. Also, the inflammatory potential of football player’s diet was lower, and they have more antioxidant potential of diet. It seems that following an anti-inflammatory and antioxidant-rich diet may be effective due to impact on reducing oxidative stress. Key words: Oxidative stress, 8-hydroxy-2'-deoxyguanosine, F2a-isoprostane, dietary inflammatory index, dietary antioxidant capacity, football.