Evaluation of mmp9 gene expression in tissues with cholesteatoma and its healthy margin

Abstract

Cholestatoma is a benign lesion that gradually spreads causing erosion of the temporal bones. This erosion causes the analysis of adjacent bone structures. This mass usually begins to form deep in the ear near the eardrum and grows toward the middle and inner ear. Ear cysts are not cancerous, but if left untreated can cause problems such as hearing loss. Studies have shown that an obvious imbalance in the regulation of MMPs in cholesteatoma leads to the destruction of the extracellular matrix by regulating increased MMP gene expression and decreasing MMP inhibitors. MMD-9 destroys extracellular matrix proteins. The aim of this study was to evaluate the expression of MMP9 gene in patients with cholesteatoma.

Methods: This research was conducted during 2019-2021 in Imam Reza Hospital in Tabriz. In this study, 40 samples of tumor tissue and 40 samples of tumor margins of patients with cholesteatoma were examined. RNA was extracted from the target tissues by the extraction kit (Trizol), cDNA was synthesized from it, then Real Time PCR was performed and finally statistical analysis was performed.

Results: In this study, 40 samples of tumor tissue and 40 samples of tumor margins of patients with cholesteatoma were examined. The expression level of MMP-9 in cholesteatoma group is significantly higher than the control group. (P = 0.0011) ROC analysis showed that the concentration of MMP-9 is optimal at 1 1.145 and has a sensitivity of 67.5% and a specificity of 84.62%. . (p = 0.001 and CI95% = 0.063-0.86)