The effect of sericin on heat shock protein 60, 90 and TLR2, TLR4 induced by chronic heat stress in male mice

Abstract

Heat stress followed by heat stroke, which is caused by an increase in body temperature to more than 40 degrees Celsius, as a medical emergency affects many people in work situations and weather conditions. May cause irreversible neurological and cognitive damage. Materials and methods: In the present study, 75 male albino mice in the weight range of 28-30 grams were used. The mice were divided into 5 groups of 15, including the following groups: control group, heat stress group, heat stress group + sericin 100, heat stress group + sericin 150, heat stress group + sericin 200. After induction of heat stress, the expression level of TLR2, 4 was evaluated by Real Time PCR and the expression of hsp60 and hsp90 proteins by western blot. Results: The expression level of TLR2 and TLR4 receptors increased significantly with the passage of time as a time course of stimulation. Western blot results also showed that the expression level of HSP60 and HSP90 also increased.