Evaluation of FIS-1 gene expression in tumor tissue and marginal tumor of Breast cancer patients

Abstract

Cancer is known as the second cause of death after heart diseases and disease (22%), its incidence depends on factors such as age, genetic background and gender. Conventional cancer is caused by aberrant cell cycle progression and incomplete apoptosis due to activation of radio-oncogenes and inactivation of tumor suppressor genes. According to published statistics, breast cancer is one of the most common cancers among women. According to the statistics of the World Health Organization, 1 out of every 8 to 10 women with breast cancer has the FIS-1 gene, which plays a role in the division and fission of mitochondria, and since this action is involved in the mitochondrial pre-apoptotic pathway, it can play a slow role in the investigation. Apoptotic pathways of cancer should be considered. In this study, the level of expression of this gene in breast cancer patients has been investigated. Methods: In this research, 50 samples of your tissue tumore and 50 tumor margin samples of cancer patients were examined. We extracted RNA from the tissues of interest by the extraction kit (Trizol), synthesized cDNA from it, then performed Real Time PCR and finally performed statistical analysis. Results: The sample size was 50. The average age was 56.7 years, 21 samples were under 55 years and 29 samples were over 55 years. The obtained results have determined that there is a significant relationship between the expression of the FIS-1 gene in cancerous tissue and healthy tissue. Also, the level of expression of this gene was higher in patients with higher stages and lymphatic involvement. This study suggests the high potential of FIS-1 expression as a diagnostic biomarker in breast cancer patients.