The effect of hydroalcholic extract of Fumaria parviflora(isoquinoline) on sperm parameters and testicular dameage induced by varicocele in adult male rat

Abstract

A varicocele is primarily defined as the abnormal enlargement and and twisting of the pampiniform venous plexus in the spermatic cord, which leads to an increase in oxidative stress and a decrease in antioxidant capacity. The consequence of varicocele is a decrease in the quality of sperm parameters and a defect in the process of spermatogenesis. Recent studies indicated that the treatment with antioxidant agents has protective effects against the formation of reactive oxygen species (ROS). The aim was to investigate the Protective effect of Fumaria Parviflora on tissue damage and oxidative stress induced by varicocele in adult rat testis Materials & Methods: Animals were randomly divided into 4 groups; control group subjected to a sham and 3 treatment groups comprising varicocele without treatment (Vcl), varicocele followed by the treatment with FP (VCL+FP) and treatment done by FP alone. After treatment period (56 days), the level of oxidative stress markers was measured in plasma and also the expression of Bax and Bcl2 were examined by RT-qPCR method. As well as the sperm parameters and srum hormone level was assessed.

Results: The Johnsen score, along with the epithelium thickness and the seminiferous tubules diameter, notably increased in varicocele group after intake the FP as compared to varicocele group. The levels of testosterone, LH, FSH and antioxidant enzyme were remarkably increased in therapy groups. The MDA levels and expression of Bax were declined in the treatment groups. As well as the expression of Bcl2 increased in treated groups.