The effect of carvacrol on lung inflammation of high-fat diet- induced obase male rats

Abstract

To date, beneficial effects of carvcrol have been shown in different inflammatory condition. Considering the inflammatory entity of obesity and lung disorders, this study was conducted to evaluate the effect of carvacrol on the inflammation as well as pathological changes in lung tissue of high-fat diet (HFD) male rats. Methods: In this study, 24 adults male Wistar rats weighing 160 ±10 g were randomly divided into three groups; Control group (C), high-fat-diet (HFD), and high-fat-diet + Carvacrol (HFD+Carva). In HFD groups, rats were received HFD for 3 months. In HFD+ Carva group, 50 mg/kbw carvacrol was injected at every two days in the last month intaperitoneally. Finally, At the end of the third month, animals were sacrificed and their lung tissues were taken. The expression of VCAM-1, ICAM-1, IL-1β, IL-6 and IL-10 was investigate using real-time PCR assay. Protein concentration of TNF-α in bronchoalveolar lavage (BAL) fluid were measured with ELISA kits. Results: Data showed pathological injuries in lung tissue of HFD groups coincided with up-regulation of VCAM-1, ICAM-1, IL-1β and IL-6 and down-regulation of IL-10 compared to the control group. Carvacrol administration had potential to inhibit HFD-induced injuries in lung tissue