| dc.description.abstract | Determining the exact time of window of implantation (WOI) in individuals with RIF is necessary for successful implantation. The present study aimed to compare the effects of letrozole and/or calcitriol on the expression of receptivity markers in endometrial epithelial cells (EECS) of RIF patients and healthy fertile women.
Methods: Endometrial tissue was obtained from RIF patients (N = 5) and healthy women (N = 5) in the proliferation phase. EECs were isolated and cultured in a fibrin scaffold in 10 groups. The cells were treated with letrozole and/or calcitriol in three types of treatment. To check the proliferation and migration of cells in the scaffold, immunofluorescent and H&E staining was used, and gene expression was checked using real-time PCR.
Results: This study showed that the expression level of pre-implantation genes (NR3C1, LIF HOXA10, miR-30d, HOXA11, Integrin αv, Integrin β3) in RIF patients was lower than healthy women and the expression level of anti-implantation genes (BCL6, miR- 145, miR-23b and miR-494) were higher in RIF patients than healthy women. In all three types of treatment (letrozole, calcitriol and co-treatment with letrozole and calcitriol), the expression level of pro-implantation genes (HOXA10, HOXA11, Integrin αv, NR3C1, miR-30d) increased except for LIF and Integrin β3 in RIF patients and healthy women. In addition, in all three types of treatment (letrozole, calcitriol and co-treatment with letrozole and calcitriol), the expression level of anti-implantation genes (miR-145, miR-23b, miR-494 and BCL6) in RIF patients and healthy women decreased. | en_US |
