Evaluation of microRNA-125 and microRNA-26a expression levels in peripheral blood effects on the prognosis of patients with covid 19

Abstract

The pathogenesis of Coronavirus 2 (SARS-CoV-2) acute respiratory syndrome infection and virus-host interactions during COVID-19 is not yet fully understood. MicroRNAs are small, non-coding RNA molecules that bind to the 3 'untranslated region of target RNAs and play a key role in regulating gene expression. MiRNAs have been shown to be deeply involved in regulating the expression of cytokines, chemokines, and growth factors, which are directly related to cytokine release syndrome (CRS) in COVID-19. Therefore, in this study, we decided to investigate the relationship between changes in the expression level of microRNA-125 and microRNA-26a in peripheral blood in the prognosis of patients hospitalized with Covid-19. Method: Samples were taken from patients with Covid-19 who were hospitalized in Imam Reza Hospital (50 patients in the ward and 50 patients in the ICU). The expression of miR-26a and miR-125 was measured by RT-PCR and between the two groups. compared. Results: MiR-26a and miR-125 gene expression was significantly lower in patients with poor prognosis compared to patients with good prognosis (p less than 0.001 and p = 0.006 for miR-125 and miR-26a, respectively). In addition, the predictive power of miR-26a and miR-125 in the prognosis of patients with Covid-19 was evaluated which was statistically significant (level below ROC = 0.97, p less than 0.001 for miR-125 and level below chart ROC = 0.98, p less than 0.001 for miR-26a).