The effects of red blood cells lysate on serum triglycerides fatty acid composition

Abstract

Serum triglycerides play an important role as energy-generating molecules in the transfer of lipids from the drug and digestive system to the peripheral tissues. The type and amount of fatty acids participating in these serum triglycerides affect the biochemical and biophysical properties of these lipids. Hemolysis is defined as the destruction or complete removal of red blood cells from the bloodstream before their 120-day lifespan.

Materials and methods:

This study was done experimentally on human samples. In this study, serum samples and red blood cells were used. A pair of serum samples and erythrocyte lysate with different concentrations were mixed according to the determined ratio. Separation of triglyceride fraction was done using thin layer chromatography method and methyl ester of fatty acids was done in acidic environment using methanol. The composition of fatty acids was determined using gas chromatography method.

Results: The average values of the percentage of saturated, monounsaturated and polyunsaturated fatty acids of the triglyceride fraction in serum containing lysate were investigated at two temperatures of 4oC (control) and 37oC. As it was observed, the total of monounsaturated fatty acids showed a significant decrease at the temperature of 37oC compared to the control group. Also, the amount of myristate and pentadecanoate fatty acids at 37oC temperature showed a significant decrease compared to the control group. In contrast, stearate fatty acid showed a significant increase compared to the control group. Also, no statistically significant differences were observed regarding trans-palmitoleate, cis-palmitoleate and oleate fatty acids between the studied groups. But the fatty acid of arachidonate (20:4) showed a significant increase in the temperature of 37oC compared to the control group.