Evaluation of exosomes biogenesis in the pulmonary tissue of ovalbumin-sensitized male rats

Abstract

Previous studies have shown that the occurrence of inflammation can alter exosomes biogenesis. Considering the complexity of asthmatic niche, the current experiment was conducted to investigate exosomes biogenesis in the pulmonary tissue of ovalbumin-sensitized male rats. Methods: Sixteen male rats were randomly divided into two experimental groups; Control and the ashmatic groups. After induction of asthma, expression of IL-4, -10, Alix, Rab27b, CD63 was monitored in lung tissue. Pathological injureis was monitored using Hematoxylin and Eosin staining. In addition, acetylcholinesterase activity was measured BALF followed by the evaluation of CD63 synthesis in lung tissue. Results: Our finding showed an increase IL-4 and the suppression of IL-10 in asthmatic lung tissues compared to the control samples. Histological examination revealed abberant remodeling in asthmatic condition. Transcritopm of all exosome-related genes Alix, Rab27b, and CD63 was up-regulated in asthmatic group realted to healthy group. In line with these conditions, protein levels of CD63 was significantly induced under asthmatic condition coincided with acetylcholine esterase activity.