Investigating the impact of combined silencing of CD73 and EGFR1 on cancer cell growth

Abstract

Neutralizing the inhibitory effect of immunosuppressive factors on tumor microenvironment is one of the effective ways for cancer immunotherapy. Studies have shown that the expression of CD73 and EGFR1 factors in tumor microenvironment at the level of cancer cells is significantly increased and increases the growth of cancer cells and suppresses anti-tumor immune responses. Therefore, inhibiting these two factors simultaneously can inhibit the growth of cancer cells. For this purpose, in this study, we used siRNA-loaded biocarriers to inhibit the expression of these molecules. Materials and Methods: In this study, superparamagnetic iron oxide (SPION) nanoparticles coated with thiol chitosan and trimethyl chitosan and TAT peptide were generated and loaded with specific siRNAs against CD73 and EGFR1. The physicochemical properties of nanodrates and their potency in suppressing the expression of target genes were also investigated. Results: The results showed that the synthesized nanoparticles had a size of about 133 nm, with a dispersion coefficient of less than 0.3 and a zeta potential of about 25. Appropriate physicochemical properties of nanoparticles caused their effective uptake by cancer cells and significantly suppressed the expression of target genes.