Evaluation of changes in the expression of molecules of immune checkpoints of cordycepin-treated colorectal cancer cells

Abstract

Tumor escape from the immune system is one of the most important signs of cancer progression. Tumors have the ability to induce the expression of molecules related to immune system checkpoints to inhibit anti-tumor responses. Immunosuppressive molecules are one of the most important obstacles in cancer treatment. These molecules are expressed on cancer cells and bind to their specific receptors on immune system cells and inhibit these cells by activating signaling pathways. CD80 and PD-L1 genes are immune system suppressor genes that are expressed on tumor cells and bind to their receptors B7-1 and PDL-1 on immune system cells, thereby preventing immune system responses. Other molecules related to system checkpoints include CD86. The expression of these genes is suppressed by miRNAs that control them, and they are also used as biomarkers of response for treatment. The aim of this study is to investigate the expression of these genes and their controlling miRNAs in cordycepin treatment. Materials and methods: HT-29 cell line were treated with Cordycepin for 24 hours after they reached a density of 80%. Then cellular RNA was extracted and finally cDNA synthesis was performed. Finally, the expression level of genes related to escape from the immune system and miRs controlling their expression were investigated by quantitative real-time PCR. Findings: Our observations showed that cordycepin decreases the expression of immune checkpoint