The evaluation of CD73, CD39 and HIF-1α expression in women with preeclampsia compared with healthy pregnant women

Abstract

The Preeclampsia (PE) molecular mechanisms are not fully revealed and different biological processes are involved in the pathogenesis of PE. We aimed to evaluate adenosine and hypoxia-related signaling molecules in PE patients in the current study. Methods: Decidua tissue and peripheral blood samples were taken from 25 healthy pregnant and 25 PE women at delivery time. CD39, CD73, and Hypoxia-inducible factor-alpha (HIF-α) were evaluated in mRNA and protein level using real-time PCR and western blotting techniques, respectively. Also, miR-30a, miR-206, and miR-18a expression were evaluated by real-time PCR. At last, secretion levels of IGF and TGF-β in the taken serum of blood samples were measured by ELISA. Results: Our results revealed that Expression of CD39 is decreased in PE cases versus healthy controls at mRNA and protein levels (p=0.0003 for both). CD73 and HIF-α showed an increased level of expression in PE patients at RNA and protein status (p=0.0157 and p<0.0001 for protein evaluation of CD73 and HIF-α, respectively). The miRNA-30a (p=0.0037) and miR-206 (p=0.0113) showed elevated expression in the decidua of the PE group. The concentration of secreted IGF-1 (p=0.0002) and TGF-β (p=0.0101) in serum samples of PE cases compared to the healthy group were decreased.