Evaluation of diagnostic value of plasma Irisin in pediatric acute appendicitis

Abstract

Appendicitis is the most common cause of abdominal pain of need urgent surgical intervention and the appendix must be excised immediately. Lifetime incidence estimated about 7 to 9 percent. Acute appendicitis in children is so important and can be difficult to diagnose. Nowadays, it is identified that conservative treatment and follow up of pediatric patients with simple appendicitis is preferred, in contrast to the surgery. In addition to history, physical examination and clinical scoring systems, physicians are looking for a new biomarker in laboratory investigations, with high accuracy in acute appendicitis diagnosis. Recently, some new protein molecules are identified by using precision medicine methods. One of the most important of these proteins, is a myokine that named as Irisin, with variety of performances that investigated recently. We evaluated the diagnostic value of Irisin in pediatric acute appendicitis and distinguishing it from nonspecific abdominal pain in this study. Materials and methods: We studied 90 children between 2 to 14 years old that accepted in Tabriz Pediatric Hospital emergency department with primary diagnosis of acute appendicitis, between January and Jun of 2021. Irisin measurement is done by Human ELISA kit. Pathology slides of processed appendectomy specimens are stained by hematoxylin-eosin and are studied by light microscope. Statistical analysis is performed by SPSS software.

Results: 90 pediatric patients that enrolled to our study, include 54 boys and 36 girls with mean age of 8.65 yrs. We had 60 acute appendicitis cases, confirmed by histopathologic study of appendectomy specimen; and 30 cases with nonspecific abdominal pain. Plasma Irisin level is significantly high in patients with acute appendicitis (P<0.001). Sensitivity and specificity of Irisin in acute appendicitis with cut off point=7.07 in ROC curve, are 82% and 83% respectively.