Development of dispersive solid phase microextraction method using magnetic adsorbent to determine the amount of vitamin B1 in infant formula

Abstract

Introduction: Considering the importance of vitamin B1 in the development of infants and children, today regulatory and medical institutions need new and more accurate methods to measure the level of thiamine in food and pharmaceutical supplements.Objective: To develop an accurate, and low-cost method to detect and measure the amount of thiamine in infant formula supplements available in the market and compare it with the manufacturer's claim on the product label.Methodology: Firstly, the parameters of thiamine chromatography separation method were selected. In the next step, after the synthesis of GO-Fe3O4 adsorbent, the effective parameters in thiamine extraction were investigated and optimized by one-parameter-at-a-time method, including the amount of adsorbent, the type and volume of desorption solvent, absorption and desorption time, and pH. It was the environment. Then thiamine analysis was done in different brands of powdered milk with the proposed method. Findings: to draw a calibration chart, B1 standard solutions were prepared by the optimized dispersive solid phase microextraction method and the linear range was drawn at 0.07-80 μg/g using a liquid chromatography-mass spectrometry device. Precision was better than 4.1% and accuracy was in the range of % 103.72-98.5, 5 samples of powdered milk with different brands and types were purchased and analyzed. Discussion and conclusion: After measuring the amount of thiamine in each product, it was observed that in 3 samples the claim on the product label was the same as the answer we received, and in two samples the answer obtained showed a different amount of thiamine compared to It was the manufacturer's claim.