Evaluating the apical microleakage of AH26, CeraSeal and Endo Plus sealer with bacterial microleakage method: in vitro study

Abstract

Introduction: One of the most common causes of failure of endodontic treatment is the lack of proper apical seal. Although different sealers have been proposed to improve the apical seal, it is very important to evaluate the capability of the sealers, especially in terms of preventing bacterial shedding, in order to introduce the best sealer in terms of the least amount of microleakage. AH26،CeraSeal and Endo Plus sealer are common and widely used seals in endodontic treatments that have not been studied in terms of apical microleakage. The aim of this study was to compare the apical microleakage of three types of sealers AH26،CeraSeal and Endo Plus sealer using bacterial microleakage method. Methods and Materials: In this in-vitro study, 148 single canal extracted permanent teeth with approximately the same root length were included in the study. The teeth were prepared by Crown Down method, modified and filtered with rotary files. 5 teeth were selected for positive control and 5 teeth for negative control. The rest of the teeth were divided into three groups of 46 and were ablationed with AH26،CeraSeal and Endo Plus sealer according to the manufacturer's method for each of the sealers. Bacterial method was used to investigate microbial microleakage. Samples were examined daily for 60 days for turbidity due to microbial microleakage of Enterococcus fecalis bacteria. Data were analyzed by Chi-square test using SPSS version 26 software. Results: In this study, although the highest bacterial microleakage belonged to the Endo group (23.9%) and the lowest to the CeraSeal sealer group (10.8%), however, there was a significant difference between There was no bacterial leakage between the three groups of AH26،CeraSeal and Endo Plus sealer (P=0.128). Conclusion: Based on the findings of the present study, all three types of sealers AH26،CeraSeal and Endo Plus have similar function in incidence of apical microleakage.