Assessment of antifungal susceptibility testing pattern of Candida species isolated from children admitted to Tabriz Children's medical center

Abstract

Introduction: Candida species are opportunistic yeasts that are capable of causing different infections among immunocompromised patients. Over the last years invasive candidiasis has become an increasing problem in neonatal and pediatric intensive care units, so obtaining adequate treatment seems necessary. Purpose: The aim of this study is to evaluate the drug susceptibility pattern of Candida species isolated from clinical samples of children and neonates admitted to different departments of Tabriz Children's medical center, to common antifungal drugs for the first time in Tabriz city.Procedure: Fluconazole, itraconazole, amphotericin B and caspofungin were diluted in the RPMI-1640 medium to yield two-times concentrations and were dispensed into 96-well microdilution trays at a final concentration. All identified Candida spp. incubated at 37 °C. The inoculum volumes were prepared by harvesting the cell from 48 h old cultures. 100 μl of cell suspensions were dispensed into 96-well microdilution trays. MIC results for all agents were determined visually following 48 h of incubation at 35 °C. Findings: In this study most of the isolated Candida species was resistance to fluconazole and amphotericin B. most of the Clinical Isolates of C.glabrata,C.albicans and C.krusei were also resistance to itraconazole, but all isolated C.kefyr species were sensitive to itraconazole. Most of the isolated candida species were sensitive to caspofungin.Conclusion: According to the results, most of the isolated Candida species were more sensitive to caspofungin than other antifungal drugs. Finally, it is emphasized that antifungal susceptibility testing is necessary to prevent treatment failure or recurrence of disease.