Application of aerogel for extraction and preconcentration of risperidone in plasma samples and the determination with liquid chromatography

Abstract

Risperidone (RISP) is belonging to the chemical class of benzisoxazole derivatives and chemically, it is 4-[2-[4-(6- fluorobenzo[d]isoxazol-3-yl)-1-piperidyl] ethyl]-3- methyl-2, 6 diazabicyclo [4.4.0] deca-1, 3-dien-5-one with molecular formula C23H27FN4O2 and CAS number 106266-06-2. In children and adolescents, risperidone is used for a range of disorders, such as behavioural problems associated with developmental disorders, disruptive behaviour disorders and tic disorders in addition to psychotic disorders.Target:In the present article we describe an innovative simple, rapid and sensitive HPLC method for the simultaneous determination of risperidone in plasma using a simple Solid– liquid extraction. The assay involves a short chromatographic run and fulfils the requirements for use in therapeutic drug monitoring.Method:A graphene oxide (GO)-based aerogel was synthesized and applied to the extraction and the determinations with the HPLC-UV. After the characterization of the produced GO-aerogel, it was utilized as a dispersive solid-phase extraction (DSPE) sorbent for risperidone (RIS) extraction from plasma samples. Aerogels are materials with a surface area-to-mass ratio and plenty of core with functional groups which can easily attach to the analytes to extract them to the second phase.Results:The suggested method determined RISP in plasma samples in the wide dynamic range from 20 ng/mL to 3 µg/mL. The LOD and LOQ of the developed method were calculated as 2.4 ng/mL and 8 ng/mL, respectively. This method has no need to precipitate plasma proteins, improving the analytical performance of the analysis. The obtained results proposed that the developed approach could be employed as an accurate method for quantification of RIS in the real plasma samples.Discuss:The designed method has the ability to determine the concentration of Risperidone in plasma and can be used to analyze plasma samples containing Risperidone.