The effects of oral methylnicotinamide and vanillic acid on production of testestrone and function of insulin in adipose and ovarian tissues in rat model of PCOS

Abstract

Polycystic Ovary Syndrome (PCOS), as a common endocrine disorder is accompanied by hyperandrogenism, insulin resistance, ovulation problems, and infertility. In this study, the effects of vanillic acid (VA) and MNAM on metabolic and endocrine abnormalities were evaluated in the adipose and ovarian tissues of a letrozole-induced rat model of PCOS. Methods and materials: In this study,twenty-four rats were received letrozole (1.5 mg/kg.b.w) by oral gavage for five weeks. Then, three experimental groups were treated by MET, MNAM, and VA through oral gavage for one month. Results:Our results showed that MNAM and VA reduced the serum testosterone levels and CYP17A1 gene expression.Furthermore, all therapeutic factors improved HOMA-IR and decreased the gene expression of resisitin after treatment. Also, MNAM and VA diminished the gene expression of visfatin in the ovarian and adipose tissues, respectively.Besides, VA significantly decreased the serum glucose and triglyceride levels. Furthermore, all therapeutic factors improved successfully activated the AMPK.