Determination of the effect of linoleic acid (LA) on the expression of immune checkpoint and immunomodulatory molecules and miRs controlling their expression in gastric cancer cells

Abstract

Tumor immune evasion is considered as an important hallmark of cancer initiation and progression. Tumors can induce the expression of immune checkpoint molecules contributing to suppress anti-tumor immune responses. The CD80, CD73, CD39 and PD-L1 genes are among the genes that suppress the immune system. They are repressed by miRNAs that control them and may also be used as part of treatment-responsive biomarkers. The aim of this study was to investigate the expression of these genes and miRNAs that control them in treatment with LA. Material and Methods: MKN-45 gastric cancer cell line was cultured in the relevant culture medium and after treatment with LA, RNA was extracted. Then cDNA was synthesized. Finally, changes in the expression of CD80, CD73, CD39, PD-L1 genes and their control miRNAs were determined by qRT-PCR. Results: QRT-PCR test changes in the expression of CD80, CD73, CD39, PD-L1 genes and miRs controlling them in treatment with LA in terms of gene expression CD80, CD73, CD39, PD-L1 showed a significant decreased, while expression of miR-142, miR-30a-5p and miR-155 significant increased.