Effects of probiotic yeast, Saccharomyces cerevisiae and Saccharomyces boulardi on proliferation and apoptosis of ovarian cancer cells

Abstract

Cancer is considered as a serious disease in recent decades. Ovarian cancer is the most common case among women after breast cancer. Therefore, it is necessary to adopt effective treatment methods with the least side effects. Researchers suggest anti-cancer effects of probiotics. Furthermore, probiotics have interesting effects on cancer. In this study, we investigated the anti-cancer effects of two important species of yeast and bacteria on ovarian cancer. Methods: The Saccharomyces bulardii was cultured in YPD medium and the Lactobacillus acidophilus was cultured in its own medium and sonication was used to break cell walls. Ovarian cancer cells were cultured in RPMI-1640 medium containing 10% FBS, 5% CO2 and 37 ° C. These cancer cells were then treated with metabolites prepared from yeast and bacteria and their antitumor effects were investigated. MTT assay was used to evaluate the toxicity of probiotics on cancer cells. The expression of genes related to migration, proliferation and cell death after treatment with yeast and bacterial metabolites in cancer cells was evaluated by qRT-PCR. Results: Cell groups treated with yeast and bacterial metabolites showed significant toxicity that the IC50 level of the yeast + bacterial metabolite group was lower than that of the yeast or bacterial metabolite group. Also, the expression of genes related to migration, proliferation and cell death after treatment with yeast and bacterial metabolites was significantly changed, which was the highest change in the group treated with yeast + bacterial metabolites.