Evaluation of anti Pseudomonas aeruginosa effect of liposomal form of imipenem / cilastatin (primaxin) in vitro condition

Abstract

Introduction: P. aeruginosa is associated with a wide range of infections. Due to the antibiotic resistance and virulence factors, it is one of the important medical challenges. Antibiotic encapsulation in liposomes is a good strategy for controlling infections caused by this microorganism. Objective: Evaluation of anti-Pseudomonas aeruginosa effect of liposomal form of imipenem/cilastatin in vitro condition. Methods: The antibiotic resistance pattern of the isolates was determined by disk agar diffusion method. The nanoliposome was prepared by thin layer and ethanol injection methods and the antibiotic was loaded into the nanoliposome. The size, morphology and zeta potential of encapsulated drug form and empty nanoliposome were obtained by SEM and DLS. The MIC, MBC and MBEC of liposomal and free drug forms were evaluated. Results: The nanoparticles were spherical with a diameter ranged from 30 to 39 nm, and the EE% in thin layer and ethanol injection methods were 97 and 98 respectively. In most of the isolates, the MIC and MBC of nanoliposome was lower than to the free drug forms, and stronger potential to eradicate the biofilm formation. Discussion: The nanoliposomes prepared in the two methods were more effective in 80% of the isolates, which had similar results to a study from Saudi Arabia. Also, in a study from Tabriz, it has been shown that the polymeric form of piperacillin/tazobactam has a high inhibitory effect, which confirms the results of our study. In general, we showed that the results of evaluating the minimum inhibitory concentration and biofilm eradication concentration of nanoliposomes had a greater antimicrobial activity than to the free drug form.