Evaluation of the role of mesenchymal stem cells and mesenchymal cells in patients with leukemia on the pattern of Th0 to T reg differentiation (CD4 + CD25 +)

Abstract

Among the important issues in the field of leukemia, the role of MSCs and Tregs in suppressing the immune system and lack of proper response. The importance of MSCs and Treg as modulators of immune cell function is well known. In the tumor environment, Tregs and MSC play an important role in the immunosuppressive ability of the immune system, which can interfere with the immune detection of tumor cells. We looked at healthy people and patients with leukemia. Materials & Methods: Healthy mesenchymal stem cells (H-MSCs) and bone marrow mononuclear cells (MNCs) were isolated in patients with isolated leukemia in DMEM culture medium containing 10% FBS. Flow cytometry was performed to confirm the nature of MSCs. Mononuclear cells were isolated from the peripheral blood (PBMCs) of healthy individuals by a filament, with MSCs of healthy and sick individuals, incubated for 72 hours, and finally the percentage of CD cells was measured by flow cytometry. Results: BM-derived MSCs of people with leukemia were similar in appearance to healthy MSCs, while having lower adhesion and slower proliferation. Our results showed that both healthy and diseased mesenchymal stem cells increased the differentiation of CD4 + CD25 + Treg cells and regulated the immune system. Also, our study showed the effect of bone marrow-derived MSCs on leukemia compared to healthy There are more Treg cells.