Determination of clonazepam in biological samples using optical methods

Abstract

Clonazepam, which is used to treat epilepsy, convulsions, and insomnia in children and adults, can cause respiratory depression, coma, and even death in some cases. Therefore, a fast, affordable and accurate method is needed to measure the amount of clonazepam in the blood. Target:In present research work, a simple, economical, accurate and fast method for determining the plasma concentration of clonazepam drug by spectrofluorimetric method has been presented. Method:A florescence-based sensor was fabricated for the quantification of clonazepam (CZP) plasma samples. Fluorescent polydopamine nanoparticles (PDNPs) was produced via a self-polymerization process in the pH of 5.8. To develop a ratiometric probe, the Rhodamine B (RhB) molecules were employed as a reference fluorescent with stable emission at the visible range. The fabricated FPNPs/RhB probe exhibits favorable selectivity towards the detection of CZP. Results: The CZP plasma levels are proportional to the enhanced fluorescence emission at concentration ranges from 0.1 to 08.1 μg/mL. This method showed low limit of detections (LLOD) of 1.18 ng/mL and 0.1 μg/mL, respectively. The developed nanoprobe showed high reliability in the detection of CZP concentrations in patients’ plasma samples. The accuracy of the probe is favorable which could be recommended for the CZP monitoring in the biological media. Furthermore, this probe is highly specific towards CZP in the presence of biologically available biomolecules, ions and co-prescribed drugs. Discuss: The designed probe has the ability to determine the concentration of Clonazepam in plasma and can be used to analyze plasma samples containing Clonazepam.