| dc.description.abstract | Molasses are viscous substance resulting from filtering sugarcane or sugar beets into sugar. They are appropriate media for microorganisms’ growth. Microorganisms which are able to utilize sucrose by certain enzymes may be present in molasses. Microorganisms are rich sources of natural antibiotics. Given the above explanations, it seems that the study and identification of native bacteria as genetic resources and national wealth of our land that have been created over the years, will be very important. Objective: This study aimed to isolate and introduce microorganisms from sugarcane molasses as a sugar-rich medium, a suitable media for bacteria’s growth (especially Bacillus strains). So that in future studies, the characteristics, capabilities and applications of these Bacteria can be studied in various fields. Materials and methods:Sample was cultured separately using four culture medium including LB culture medium, LB medium without NaCl (we used the same amount of glucose instead of NaCl), LB medium plus 5% glucose and LB medium plus 10% glucose. Genomic DNA was extracted from the isolates by Corbin method. Extracted DNAs were sequenced by a Korean sequencing company (Macrogen). For identification of bacteria, the sequences were analyzed using the BLAST program with the sequences deposited in NCBI and Gene Bank. Biochemical tests were fulfilled then.Results:Ninemicroorganisms were isolated from the molasses sample. Seven colonies isolated from LB medium (SE12, SE13, SE14, SE15, and SE16). Three colonies isolated from LB medium without NaCl plus 1% glucose (SE1, SE2, and SE6). And one colony from LB+5% glucose (SE9). No colonies grew in LB+10% glucose. Conclusion: As predicted, all of isolated colonies belong to Bacillus and Bacillaceae strains. These strains are capable of producing enzymes that hydrolyze sugar, so that they can survive in sugarcane molasses. | en_US |
