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dc.contributor.authorAli Khiyavi, Payam
dc.date.accessioned2022-08-28T10:18:34Z
dc.date.available2022-08-28T10:18:34Z
dc.date.issued2022en_US
dc.identifier.urihttp://dspace.tbzmed.ac.ir:80/xmlui/handle/123456789/67078
dc.description.abstractRecently, various studies have focused on the therapeutic potential of thymoquinone, a natural polyphenol, in various human malignancies, including osteosarcoma. However, the underlying mechanisms in the thymoquinone-mediated anti-cancer effects are still not completely understood. Therefore, the present study evaluated the effect of thymoquinone on the metotherxarte induced- apoptosis in Saos-2 cells. Methods Saos-2 cells were treated with methotrexate, thymoquinone, and combination of both, and cellular viability was assessed by MTT assay. The mRNA expression of apoptosis markers including Bax, BCL-2, and caspase-9 was evaluated via using quantitative real-time polymerase chain reaction (qRT-PCR). Results Methotrexate led to significant inhibition of cellular proliferation in a dose-dependent manner. The combination of thymoquinone and thymoquinone led to significant inhibition of proliferation in comparison to monotreatments (P<0.05). Thymoquinone also induced apoptosis through upregulating pro-apoptotic markers including Bax and caspase-9 and downregulating anti-apoptotic mediators including BCL-2. In addition, thymoquinone increased methotrexate-induced apoptosis in Saos-2 cells.en_US
dc.language.isofaen_US
dc.publisherTabriz University of Medical Sciences, Faculty of Medicineen_US
dc.relation.isversionofhttp://dspace.tbzmed.ac.ir:80/xmlui/handle/123456789/67077en_US
dc.subjectosteosarcomaen_US
dc.subjectthymoquinoneen_US
dc.subjectapoptosisen_US
dc.subjectmethotrexateen_US
dc.titleStudy the effects of thymoqinone on sensitivity of Saos-2 cancerous cells to methotrexateen_US
dc.typeThesisen_US
dc.contributor.supervisorYousefi, Bahman
dc.identifier.docno6010460en_US
dc.identifier.callno10460en_US
dc.description.disciplineMedicineen_US
dc.description.degreeMD Degreeen_US


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