Evaluation of the effect of simultaneous inhibition of STAT3 and Gp130 in 4T1 and B16-F10 cancer cell lines using graphene oxide-trimethyl chitosan nanoparticles loaded with siRNA molecules

Abstract

Tumor microenvironmental management by inhibiting the expression of effective factors in tumor growth and development and inhibition of anti-tumor immune responses is one of the effective methods in immunotherapy. Among the main factors that facilitate tumor growth, STAT3 and Gp130 factors play an important role in tumor progression and their inhibition can be a good strategy for efficient immunotherapy. Therefore, in this study, we decided to suppress cancer cells by simultaneously inhibiting STAT3 and Gp130 molecules. Materials and Methods: In this study, we used carboxylated graphene oxide nanoparticles coated with trimethyl chitosan and hyaluronate for targeted transfer of siRNA molecules against STAT3 and Gp130 molecules into two cell lines 4T1 and B16-F10. Results: The synthesized nanoparticles had suitable physicochemical properties that led to the suppression of the expression of STAT3 and Gp130 molecules. In addition, the combined treatment of cancer cells with siRNA-loaded nanoparticles significantly suppressed the expression of genes involved in survival, proliferation, metastasis and angiogenesis.