The effect of SPION nanoparticles loaded with siRNA molecule against CD73 along with BV6 on the tumor cell’s growth

Abstract

Numerous factors in tumor microenvironment lead to increased survival, proliferation and growth of cancer cells. These factors can work together to stimulate cancer growth. Among these factors, the CD73 molecule can increase the proliferation, survival, and metastasis of cancer cells by facilitating the production of adenosine in the tumor microenvironment. On the other hand, it has been shown that the compound BV6 can induce apoptosis in cancer cells by inhibiting apoptosis inhibitors. Materials and Methods: In this study, we used superparamagnetic iron oxide (SPION) nanoparticles loaded with siRNA to turn off the CD73 gene and the BV6 compound. The physicochemical properties of the nanoparticles were also investigated. The effect of combination therapy on the expression of genes involved in apoptosis and metastasis was also evaluated. Results: The nanoparticles had a size of about 133 nm, with a scattering coefficient of about 0.3 and a zeta potential of 25 mV. Addition of TAT peptide to TMC-TC-SPIONs significantly increased the uptake of nanoparticles by cancer cells. Appropriate physicochemical properties of nanoparticles and controlled release of siRNA make these nanoparticles an ideal agent in gene therapy. SiRNA-loaded nanoparticles could well suppress CD73 expression in cancer cells. Combination therapy also significantly induced apoptosis-promoting genes and suppressed genes involved in metastasis.