The effect of voluntary exercise on plasma levels of Retinol-Binding Protein 4, IGF-Binding Related Protein and metabolic parameters in high fat diet male rats

Abstract

Today, changes in lifestyle and diet cause a series of diseases in society. A high-fat diet can increase the chances of obesity and cardiovascular disease in addition to the development of insulin resistance in the development and progression of diabetes. Considering Retinol binding protein 4 (RBP4) produced by fat and liver cells, which can cause insulin resistance, and the relationship between IGF-binding protein (IGFBP) and the process of insulin resistance, we decided to evaluate the effect of exercise. Deliberately examine these factors and metabolic parameters.

Materials & Methods: In this study, 28 male Wistar rats were randomly divided into 4 groups (n = 7). 1) Control group: without any intervention. 2) High-fat diet group: In this group, the animals received a high-fat diet for 10 weeks. 3) Voluntary exercise group: In this group, the animals did voluntary exercise for 10 weeks. 4) High-fat diet group and voluntary exercise: In this group, the animals received a high-fat diet and exercised voluntarily for 10 weeks at the same time. Voluntary exercise was performed for 10 weeks by placing the animal in a special cage with a rotating wheel. At the end of the intervention, the animals were anesthetized and blood samples were collected to measure the relevant proteins and metabolic factors (TG, HDL and cholesterol). Results: Compared with the control group, while the high-fat diet significantly increased cholesterol, triglycerides, IGFBP and RBP4 (p <0.05), exercise alone did not affect these parameters and only combined with the high-fat diet, significantly reduced them. (p <0.05).